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贴壁细胞的脂质体转染关键词： HYPERLINK :.ebioetag.phptag=D6CACCE5 质体 HYPERLINK :.ebioetag.phptag=CFB8B0FB 细胞 HYPERLINK :.ebioetag.phptag=CCF9B1DACFB8B0FB 贴壁细胞 HYPERLINK

单击此处编辑母版标题样式单击此处编辑母版文本样式第二级第三级第四级第五级细胞转染技术原理：细胞生物学研究证明细胞膜的磷脂双分子层可视为一种电容其静膜电位(Vm)约为l00mV导电性很差当把细胞置于外加电场中时会使膜电位升高双分子层两端电压形成差势随着外加电场电压升高细胞膜被压缩变薄当膜电压升到一定数值时膜被击穿 可逆击穿：击穿小孔可在一定时间内复原不可逆击穿：击穿小孔不可修复处理细胞成活率低操作程

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细胞转染实验原理仪器试剂和操作步骤外源基因进入细胞主要有四种方法：电击法磷酸钙法和脂质体介导法和病毒介导法电击法是在细胞上短时间暂时性的穿孔让外源质粒进入磷酸钙法和脂质体法是利用不同的载体物质携带质粒通过直接穿膜或者膜融合的方法使得外源基因进入细胞病毒法是利用包装了外源基因的病毒感染细胞的方法使得其进入细胞但是由于电击法和磷酸钙法的实验条件控制较严难度较大病毒法的前期准备较复杂而且可能对于细

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