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Protocol for western bloting Ji xiong at joint lab 07-9-7实验准备:分离胶10gelVolume浓缩胶VolumeH2O ×2ulH2O 700×2ul30×2ul30×2uul10ul10u 5mltotel 2ml电极缓冲液1×10×25mM SDS1g10gfinal volume1L1L转膜缓冲液volume甘氨酸甲醇10
Cleaning Validation Protocol for PharmaceuticalsIntroductionThe Validation of the Cleaning Procedures is establishing documented evidence that the procedure is effective and capable for removing
实验方法原理Western免疫印迹,是将蛋白质转移到膜上,然后利用抗体进行检测的方法。对已知表达蛋白,可用相应抗体作为一抗进行检测,对新基因的表达产物,可通过融合部分的抗体检测。与Southern或Northern杂交方法类似,但Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗。经过分离的蛋
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Network Working Grou
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CMC:AMODEL
Limited Flooding Pr
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