实时PCR实时PCR(real-time PCR),属于定量PCR(Q-PCR)的一种,以一定时间内DNA的增幅量为基础进行DNA的定量分析。 real time PCR 的定量使用荧光色素,目前有二种方法。一种是在ds DNA中插入特异的荧光色素,例如SYBR Green荧光染料;另一种使用一种能与增幅DNA序列中特定寡核酸序列相结合的一种荧光探针(probe),如Taqman探针。、两种方
编号:2-5主题:Real-Time PCR概述:Real-Time PCR 技术,又称实时定量荧光PCR(real-time quantitative PCR),是指在PCR反应体系中加入荧光基团,利用荧光信号累积实时监测整个PCR进程,最后通过标准曲线对未知模板进行总量分析或通过Ct值对模板进行相对定量。实时荧光定量PCR技术于1996年由美国Applied Biosystems推出,该
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单击此处编辑母版标题样式单击此处编辑母版文本样式第二级第三级第四级第五级Real-Time Windows Target主讲教师姜洪洲机电学院快速控制原型Rapid Control Prototyping The Process of implementing a control design quicklyusing RCP software tools and RCP hardware t
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マスタ タイトルの書式設定线性图谱 线性期 RCT= RB X0(1 e)CT Rs lg (RCT -RB) = lg X0 CT lg (1 e) lg Rs CT lg (1 e) = - lg X0 lg (RCT -RB) –lgRs S log( 即 CT = - k lg X0 b(线性方程) CT = - k lgX0 b R Q 5 绝对定量 Cts
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